Thermal Microplate Seal Selection Guide

The Heat Sealing Process

Microplates:  Factors such as plate design, material, manufacturer consistency, and various other physical features affect seal quality. Be sure to only use microplates that are SBS compliant. Here are some other factors to consider when selecting a compatible microplate for thermal sealing.

  • Raised rims around each well typically produce the best results, though they are not required.
  • Skirtless and half-skirt PCR plates require plate supports.
  • Polypropylene microplates offer the most effect and reliable heat seal, though we offer other heat seals that are designed to be compatible with specific polymer substrates such as polystyrene and COC.
  • Avoid acrylic microplates because they are not compatible with thermal microplate sealing (See Adhesive Seals for sealing onto acrylic plates).

Seals: Films or foils are welded to microplates using heat sealers to produce a secure seal, normally to raised rims around the individual wells of the microplate. Heat seals are available in carefully tested materials that are suitable for peeling, piercing, storage, transport, and temporary or long-term sealing. Seals that can withstand sub-zero or high temperatures are also available.

Thermal Microplate Seal Types

ClearASeal Peel™ Heat Sealable Seal

ClearASeal-Peel
Clear peelable film with moderate solvent resistance.

Features: Film, clear, peelable, good optical clarity, moderate solvent resistance, peelable, non-pierceable.
Compatibility: Polypropylene (PP), Polyethylene (PE), Polystyrene (PS) & Cyclic Olefin Copolymer (COC) plates.
Seal integrity range: -80°C to 80°C.
Application: qPCR, short term compound storage.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

ClearASeal Pierce™ Heat Sealable Seal

ClearASeal-Pierce
Clear pierceable film ideal for use with ABI 3730 sequencer.

Features: Film, clear, non-peelable, good optical clarity, moderate solvent resistance, pierceable.
Compatibility: Polypropylene (PP), Polyethylene (PE), Polystyrene (PS) & Cyclic Olefin Copolymer (COC) plates.
Seal integrity range: -80°C to 80°C or 110°C with pressurised PCR heated lids.
Application: Recommended for use with the Abi 3730 Sequencer as the thinner structure pierces more easily.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

ClearASeal Weld™ Heat Sealable Seal

ClearASeal-Weld
Clear strong bonding film ideal for water thermal cyclers.

Features: Film, clear, good optical clarity, solvent resistance, permanent seal, non pierceable, non peelable.
Compatibility: Permanent seal to Polypropylene (PP).
Seal integrity range: -80°C to 110°C.
Application: qPCR, PCR, (water bath thermal cycling), storage, sample inspection, disposal of hazardous materials, use with DMSO.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

PeelASeal Foil™ Heat Sealable Seal

PeelASeal-Foil
Peelable foil laminate for polypropylene plates.

Features: Foil laminate, good liquid barrier, moderate solvent resistance, peelable (from -80°C freezer), non-pierceable.
Compatibility: Polypropylene (PP) plates.
Microplate Heat Seal integrity range: -80°C to 110°C.
Application: PCR, low temperature, short term compound storage, short term room temperature compound storage (less than 5 days).
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

PeelASeal DMSO Foil™ Heat Sealable Seal

PeelASeal - DMSO Foil
Peelable DMSO resistant foil for Polypropylene plates.

Features: Foil laminate, good liquid barrier, high solvent resistance (at high temperatures) peelable, non-pierceable.
Compatibility: Polypropylene (PP) plates.
Seal integrity range: -80°C to 80°C.
Application: Low temperature and ambient temperature storage with DMSO & other solvents.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

PierceASeal Foil™ Heat Sealable Seal

PierceASeal-Foil
Pierceable foil seal with good solvent resistance.

Features: Foil, high solvent resistance including DMSO, non-peelable, multiple sealing/re-sealing permissible, easy sealing surface identification, pierceable.
Compatibility: Polypropylene (PP), Polystyrene (PS).
Seal integrity range: -20°C to 120°C.
Application: PCR, compound storage, sample shipping.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

PierceASeal Foil PS™ Heat Sealable Seal

PierceASeal-Foil
High grade foil, pierceable and peelable to polystyrene plates.

Features: Foil, good solvent resistance including DMSO, peelable – polystyrene only, pierceable, multiple sealing and re-sealing permissible, easy non-sealing surface identification.
Compatibility: Polypropylene (PP), Polystyrene (PS).
Seal integrity range: -20°C to 110°C.
Application: PCR low temperature compound storage, short term room temperature compound storage.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

ThermASeal Foil™ Heat Sealable Seal

ThermASeal - Foil
Strong bonding foil to polypropylene, ideal for water thermal cyclers.

Features: Foil laminate, strong bonding, good solvent resistance including DMSO, peelable, non-pierceable.
Compatibility: Polypropylene (PP), Polystyrene (PS).
Seal integrity range: -200°C to 110°C.
Application: PCR, specifically water thermal cyclers. Storage of solvents and other organics, including acids and alkaline. Long term storage. Transportation at low temperature.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

PeelASeal Black Foil PS™ Heat Sealable Seal

PealASeal - Black Foil
Peelable low fluorescence coated foil for light sensitive assays.

Features: Coated foil, low fluorescence, peelable, non-pierceable, typically used with black MicroPlates.
Compatibility: Black MicroPlates, Polypropylene (PP), Polystyrene (PS).
Seal integrity range: -20°C to 80°C.
Application: Fluorescent and light sensitive assays.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

Gas PermASeal™ Heat Sealable Seal

Gas-PermASeal
Non-woven gas permeable peelable seal for cell & seed culture.

Features: Opaque, non-woven, porous, gas permeable, barrier to solid contaminants, pierceable, peelable. Seals to polypropylene and polystyrene plates. Pierceable. Peelable. Not certified free from nucleases and DNA.
Compatibility: Polypropylene (PP), Polystyrene (PS).
Seal integrity range: -20°C to 80°C.
Application: Short term incubation, agriculture and seed storage, insect storage, cell culture.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

ClearASeal Perf™ Heat Sealable Seal

ClearASeal-Perf
Clear perforated gas permeable film for cell & seed culture.

Features: Clear perforated gas permeable film, non-peelable, good optical clarity, moderate solvent resistance.
Compatibility: Polypropylene (PP), Polyethylene (PE), Polystyrene (PS) & Cyclic Olefin Copolymer (COC) plates.
Seal integrity range: -80°C to 80°C or 110°C with pressurized PCR heated lids.
Application: Short term incubation, agriculture and seed storage, insect storage, cell culture.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

Gas PermASeal Clear™ Heat Sealable Seal

ClearASeal-Perf
Clear, gas permeable heat seal.

Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

PeelASeal Foil SUPER™ Heat Sealable Seal

PeelASeal-Foil
A ‘stick to all’ peelable, foil laminate heat-seal film which is suited for all plate types – PP, PS and COC. The film has a good liquid barrier and high resistance to solvents (DMSO). It is peelable (from -80°C freezer) and is non-pierceable.

Features: Peelable, non-pierceable seal with a good liquid barrier and high resistance to solvents.
Compatibility: Polypropylene (PP), Polystyrene (PS), Cyclic Olefin Copolymer (COC) Plates.
Seal integrity range: -80°C to 110°C
Application: PCR, low temperature, short term compound storage, short term room temperature compound storage (less than 5 days).
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.

PierceASeal Foil SUPER Heat Sealable Seal

PierceASeal-Foil
A ‘stick to all’ surfaces, pierceable foil seal with easy sealing surface identification and moderate solvent resistance. The film is peelable and pierceable.

Compatibility: Polypropylene (PP), Polystyrene (PS), Cyclic Olefin Copolymer (COC) Plates.
Seal integrity range: -80°C to 80°C.
Application: PCR, compound storage, sample shipping.
Sterile/non-sterile
Apply these Seals with the following Equipment:
* Efly, Kseal, 4s2
** Wasp, Thermo ALPS300/3000, Kube, Flexiseal, Chameleon, REMP (PHS)
*** Agilent VII Plateloc, REMP (LHS/SHS)

Contact us to request more information.